Mycobacterium
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Transcript of Mycobacterium
Universidad de Cuenca
Facultad de Ciencias Agropecuarias
Escuela de Medicina Veterinaria y Zootecnia
Mycobacterium
María Estela Encalada Paredes
Bacterial cell wall structure
Gram
+
Gram -
Mycobacterium
Lipid Peptido- Lipid + Porins Mycolate Acyl LAM arabino-
Bilayer glycan LPS Lipids Lipo-arabino galactan
mannin
Tuberculosis en linfonódulos medianísticos medios. Los linfonódulos están
aumentados de tamaño y muestran múltiples tubérculos pequeños de tipo caseoso
OTRAS PRUEBAS
• ELISA
• Ensayos de transformación linfocitaria
• Ziehl Neelsen
• Cortes histológicos
• Cultivos
• Perfil bioquímico
• Cromatografía (técnicas analíticas)
• PCR Polymerase chain reaction (técnica
molecular)
• Sistemas automatizados
Johne’s Disease:
What is it ?
Pronounced: “YO - knees”
A chronic bacterial infection .
Affecting primarily the intestinal tract but leads to infection throughout the body.
Incurable chronic wasting condition.
Affects: cattle, deer, bison, sheep, goats, camels rarely pigs & horses
Long incubation period: 2-10 years
Discovered in 1895 by Johne and Frothingham
Mycobacterium paratuberculosis
Bacterium
Related to tuberculosis
Very resistant/ hardy
River water 165 days
Tap water 520 days
Freezing temperature 245 days
Slurry storage 252 days
Survives in the feces for up to 1 year
Survives commercial pasterurization - 3% of milk cartons
Slow growing 3-4 months in lab
Shedding of the organism is sporadic
Johne’s Signs?
Signs:
95% of infected cattle show NO Signs !!
5% show weight loss, then intermittent diarrhea
Clinical signs in animals > 2 years old
Culled before diagnosed (10% of culls)
Herd signs:
One animal with chronic diarrhea then another several months later.
Bacilos de M. paratuberculosis teñidos mediante Ziehl-Neelsen en frontis
de mucosa intestinal aparecen agrupados en forma características
A. Íleon normal y en el otro caso mucosa con
gruesos pliegues transversos difícil de suavizarlos al
ser estirados.