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Producción Asparaginasa
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Transcript of Producción Asparaginasa
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7/24/2019 Produccin Asparaginasa
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PONTIFICIA UNIVERSIDAD CATLICA DE VALPARAISO
FACULTAD DE INGENIERA
ESCUELA DE INGENIERA BIOQUIMICA
ICB 540
PRODUCCION DE METABOLITOS
ASPARAGINASA
Alumnos P!m"l! Co#$"s
Cl!u%&! M!ll'!
N!(!%"$ Ro%#')u"*
P#o+"so# Cl!u%&! Al$!m!no
,ul&o B"##&os
A-#&l .0/5
Introducc
in
10% 70
Disc.
Bibliog,
50% 60
Conclusi
n
30% 65
Referenci 10% 50
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INDICE
CAPITULO /
INTRODUCCIN
CAPITULO .
./ RELEVANCIA 1 APLICACIN
.. PRO1ECCIONES DE LA PRODUCCION
.2 PROCESO DE PRODUCCION
CAPITULO 2
CONCLUSIONES
REFERENCIAS
BIBLIOGRAFIA
ANE3OS
ANE3O /
MECANISMO DE ACCION ASPARAGINASA
ANE3O .
DIFERENTES FUENTES BACTERIANAS PARA ASPARAGINASA
ANE3O 2
EVOLUCION TEMPORAL DE LA APARACION DE ASPARAGINASA II
ANE3O 4
USO INDUSTRIAL DE ACR1LAA1 EN EL MUNDO
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INDICE DE TABLAS
T!-l! / D&+"#"n$"s Fu"n$"s -!6$"#&!n!s 7!#! !s7!#!)&n!s!
INDICE DE FIGURAS
F&)u#! / Es8u"m! m"6!n&smo %" #"!66&9n !s7!#!)&n!s!
F&)u#! . E:olu6&9n $"m7o#!l %" l! !7!#&6&9n %" !s7!#!)&n!s! II "n 6ul$&:os
F&)u#! 2 Uso &n%us$#&!l %" No:o*(m"s A6#(l!;!( "n "l mun%o !l !
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4
CAPITULO /
INTRODUCCIN
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"n 7l!n$!s 6omo "n m!m'+"#os ( "s$> %&s$#&-u&%! !m7l&!m"n$" "n m&6#oo#)!n&smos@ 6omo
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un! "l&m&n!6&9n 6om7l"$! %" l! "n+"#m"%!%@ 6!$!lo)!n%o l! "n*&m! 6omo un! %#o)!
-&o8u'm&6! "+"6$&:! =G!ll!)"# "$ !l@ /?
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7"#o los n&6os %os 7#o%u6$os 8u" 7#"s"n$!n un "$"nso uso "n $#!$!m&"n$os 6l'n&6os son
!8u"ll!s "n*&m!s %"#&:!%!s %" E. coli( Erwinia=P!#H 1 "$ !l@ //?
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5
CAPITULO .
./ RELEVANCIA 1 APLICACIN
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!s7!#!)&n! "s un !m&no>6&%o no "s"n6&!l@ $&"n" #"7"#6us&9n &m7o#$!n$" "n "l $#!$!m&"n$o
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no 7u"%"n s&n$"$&*!# !s7!#!)&n!@ %"-&%o ! l! !us"n6&! %" !s7!#!)&n! s&n$"$!s! =K"!$&n) "$
!l@ /2?@ 7o# lo 6u!l !l no s"# 6!7!6"s %" 7#o%u6 "s$" !m&no>6&%o@ %"7"n%"n
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no$!# un! %" l!s 7o6!s %&+"#"n6&!s "s7"6'+&6!s 8u" 7o%"mos #"6ono6"# "n$#" 6lul!s
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!s7!#!)&n!@ no son !+"6$!%!s 7o# un! %"+&6&"n6&! &n%u6&%! %" "s$! sus$!n6&!
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6
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m"nos "+"6$os !%:"#sos
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#"6om"n%!6&on"s 7!#! #"%u6 los n&:"l"s %" "s$" 6om7u"s$o =E6 =Eu#o7"!n Com&s&on?@
.0//?
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7
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+lu&%os 8u" l!s 7os"!n@ "n "s7"6&!l l! 7#o:"n&"n$" %"Aspergillus oryzae=Gu7$!@ .0/.?
.. PRO1ECCIONES DE LA PRODUCCION
L! -s8u"%! %" nu":os m&6#oo#)!n&smos 8u" 7u"%!n s&n$"$&*!# "s$! "n*&m! ( 6on
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8u" m&"n$#!s s&)! 6#"6&"n%o "l m"#6!%o !l&m"n$!#&o %" 7#o%u6$os o#n"!%os su %"m!n%!
!um"n$!#> L! solu6&9n "s !6$u!lm"n$" u$&l&*!%! "n m>s %" 20 7!'s"s "n un! !m7l&!
)!m! %" 7#o%u6$os C#""mos 8u" "s$o %"mu"s$#! "l !l*! 8u" ! $"n&%o %"s%" su
!7!#&6&9n ( l! &m7o#$!n6&! %" l! !s7!#!)&n!s!@ 7#&n6&7!lm"n$" %"s%" 8u" l! EFSA ( l! FDA
#"6om"n%!#on ! l! &n%us$#&! !l&m"n$!#&! s"# 6ons6&"n$"s %" los n&:"l"s %" !6#&l!m&%! "n
sus 7#o%u6$os ( l! !7l&6!6&9n %" nu":!s $"6nolo)'!s 7!#! #"%u6los =FDA@ .0/2?
.2 PROCESO DE PRODUCCION
En /52 s" o-s"#:9 8u" "l su"#o %" 6o-!(o 6!us9 #")#"s&9n %" !l)unos $&7os %" $umo#"s
"n #!$on"s ( #!$!s O6o !s $!#%"@ "l !)"n$" !n$&$umo#!l "n "l su"#o %" 6o-!(! +u"
&%"n$&+&6!%o 6omo !s7!#!)&n!s! =B#oom"@ //?@ s&"n%o s$" "l 7#&m"# 7#o6"so %"
o-$"n6&9n %" !s7!#!)&n!s! 7!#! "l $#!$!m&"n$o %" $umo#"s El !n>l&s&s #":"l9 8u" un!
un&%!% In$"#n!6&on!l =UI? %" !s7!#!)&n!s! "s l! 6!n$&%!% 8u" :! ! l&-"#!# un m&6#omol %"
NJ %" !s7!#!)&n! L! %os&s um!n!@ 7u"%" #"8u"# /000000 UI o m>s 7o# %'! 7o#
7"#son!@ s&)n&+&6!n%o 8u" 7!#! l! 7#o%u66&9n %" $!l"s %os&s s"#'! n"6"s!#&o "$#!"# "l
su"#o %" m>s %" 20000 6o-!(os 7o# %'! 7o# 7"#son! =Ro-"#$s !n% J&ll@ /? C#""mos
8u" "l 6os$o 8u" )"n"#!-! "l $#!$!m&"n$o 7o# 7"#son!@ 6ons&%"#!n%o $!n$o l! 6!n$&%!% %"
!n&m!l"s %" los 8u" s" %"-'! "$#!"# "l su"#o ( "l 6os$o %" 7#o6"s!# "l m&smo 7!#! un!
%os&s %&!#&!@ "#! "6"s&:!m"n$" !l$o@ 7o# lo $!n$o "s$" m$o%o no "#! #"n$!-l"
Du#!n$" los s&)u&"n$"s !
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u$&l&*!%!s (P!#H "$ !l@1981) L!s 7#"7!#!6&on"s 6om"#6&!l"s %" !s7!#!)&n!s! m>s us!%!s
o( "n %'! son 6&%"#ol!s"@ "ls7!#@ m"%!6@ on6!s7!#@ "#8&n!s" =O#$un@ .0/.?
L! 7#o%u66&9n %" !s7!#!)&n!s! II 7o# E. colis" ll":! ! 6!-o "n 6ul$&:os 6on$&nuos "n un
m"%&o %" +"#m"n$!6&9n %u#!n$" l! $#!ns&6&9n %" 6#"6&m&"n$o !"#9-&6o ! !n!"#9-&6o =C"%!#
!n% S6;!#$*@ /? El 6u#so $"m7o#!l %" l! !7!#&6&9n %" l! "n*&m! -!o "s$!s
6on%&6&on"s s" mu"s$#! "n "l !n"o .2 Lu")o l!s 6lul!s %" E. colison #"6o)&%!s %"l
6ul$&:o 7!#! o-$"n"# un "$#!6$o !6uoso 8u" 6on$&"n" !s7!#!)&n!s!s@ 7u#&+&6!n%o (
6on6"n$#!n%o "l "$#!6$o 7o# 7#"6&7&$!6&9n +#!66&on!%! ( 6#om!$o)#!+'! To%o "s$o s"
#"!l&*! "n un m"%&o %" +"#m"n$!6&9n 8u" 6on$&"n" un! +u"n$" %" nu$#&"n$"s !s&m&l!-l" %"
6!#-ono@ ( 7!#! 7#o:""# %" un! +u"n$" %" nu$#&"n$" %" n&$#9)"no s" u$&l&*! un! 7"7$on!
7#"+"#&-l"m"n$" 7#o%u6&%! 7o# l! %&)"s$&9n 7!n6#">$&6! %" l! 6!s"'n!@ l! 6u!l s" :"n%"
-!o l! m!#6! 6om"#6&!l N !m&n! T&7o B =M!s-u#n !n% #&s$on@ /4?
Po# o$#o l!%o l! 7#o%u66&9n %" !s7!#!)&n!s!s %" Erwinias" 7#"7!#! ! )#!n "s6!l! "n
6ul$&:os 7o# lo$" ( "n un! 6olumn! %" 6#om!$o)#!+'! %" &n$"#6!m-&o &9n&6o S" #"8u&"#" un
)#!n nm"#o %" "$!7!s 7!#! !l6!n*!# l! 7u#"*! n"6"s!#&!@ 6on 6#om!$o)#!+'! %"
&n$"#6!m-&o &9n&6o 7o# lo$" L! 7#>6$&6! 6om"#6&!l !6$u!l &m7l&6! "l 6#"6&m&"n$o %" un6ul$&:o %" 6lul!s@ ( lu")o un! "$#!66&9n %" 7#o$"'n! 6#u%! "m7l"!n%o &n$"##u76&9n
!l6!l&n! ( 7#"6&7&$!6&9n 6on >6&%o El "$#!6$o #"sul$!n$" s" som"$" "n$on6"s ! l!
!%so#6&9n ( "lu6&9n %" CM6"lulos! Lu")o s&)u" un! "$!7! %" 7#"6&7&$!6&9n %" >6&%o m>s
( "$!7!s %" 6#om!$o)#!+'! u$&l&*!n%o CM ( m"%&os DEAE6"lulos! =Go;!#%@ /4?
Po%"mos !7#"6&!# 8u" l! s")u&%&ll! %" "$!7!s !6" 8u" s"! un 7#o6"so 6on un! m!no %"
o-#! &n$"ns! 7!#! l! o-$"n6&9n 7u#! %" !s7!#!)&n!s!@ #"8u&n%os" %" 6u&%!%os (
7#"6&s&on"s 7!#! 8u" s"! un 7#o6"so +&!-l" ( +!6$&-l" A%"m>s 6#""mos 8u" l!s 7#>6$&6!s
8u" s" #"!l&*!n "n "l 7#"s"n$" son 7!#! o-$"n"# m!(o#"s 6!n$&%!%"s %" !s7!#!)&n!s! !7!#$ %" %"$"#m&n!%!s 6!n$&%!%"s %" m&6#oo#)!n&smos@ #"%u6&n%os" !s' "l )!s$o %"
7#o%u6 l! "n*&m!@ !6&"n%o "6on9m&6!m"n$" m>s +!6$&-l" l! !%m&n&s$#!6&9n "n
7!6&"n$"s Po# o$#! 7!#$" 7"ns!mos 8u" "s %" 6ons&%"#!-l" &m7o#$!n6&! 6om"#6&!l 8u"
"&s$! !6$u!lm"n$" l! 7#o%u66&9n %" !s7!#!)&n!s! 7o# %os m&6#oo#)!n&smos %&+"#"n$"s@
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7"#m&$&"n%o ! 7!6&"n$"s 8u" 7#"s"n$"n "+"6$os s"6un%!#&os 6on un $#!$!m&"n$o@ un!
$"#!7&! !l$"#n!$&:!
Un! "!m&n!6&9n 6om7!#!$&:! %" E. coli!s7!#!)&n!s! 7#o%u6&%! "n USSR@ Al"m!n&! (,!79n +u" "6! 7o# =Kon%#!$@ /4? ( #":"l9 8u" l!s 6!#!6$"#'s$&6!s 6l'n&6!s %" l!
7#"7!#!6&9n "6! "n URSS ( Al"m!n&! "#!n &%n$&6os L! !66&9n !n$& l"u6m&6! %" l!
7#"7!#!6&9n "6! "n l! URSS +u" su7"#&o# 8u" ! l! "6! "n ,!79n Po# lo $!n$o Ss"
#"6om&"n%! "l uso 6l'n&6o %" !s7!#!)&n!s! "6! "n URSS ( Al"m!n&! =V"#m! "$ !l@
.00?
Un! +o#m! &nno:!%o#! %" "l&m&n!# "l 6>n6"# 8u" 6!us! l! !6#&l!m&%! %" 7!n ! s&%o%"s!##oll!%! 7o# &n:"s$&)!%o#"s %" l! "m7#"s! 8u'm&6! ol!n%"s! DSM@ u$&l&*!n%o
$"6nolo)'! %" )"n"s 7!#! %")#!%!# l! !s7!#!)&n!@ "l !m&no>6&%o l&-#" 7#"6u#so# %" l!
!6#&l!m&%!@ !n$"s %" l! 6o66&9n Es$" 7#o6"so &m7l&6! l! 6lon!6&9n %" un! 6"7! %"
!s7!#!)&n! &%#ol&*!%! 7o# !s7!#!)&n!s! %"s%" Aspergillus Nigerniger L! 6"7!
#"sul$!n$" %" l! +"#m"n$!6&9n s" 7u#&+&6! !n$"s %" s"# !
( ! un 7J %" "n$#" 0 ! 0 D!%o 8u" l!s $"m7"#!$u#!s %" 6o66&9n ! m"nu%o su-"n !
/.0 C@ "s 6on:"n&"n$" %&s7on"# %" "n*&m!s 8u" s"!n "s$!-l"s ( !6$&:!s "n un! !m7l&!
)!m! %" $"m7"#!$u#! ( 7J =S&n! "$ !l@ .0/2? En "l !s %" 20 7!'s"s &n6lu&%o C&l" s")n %!$os %" l! m&sm! "m7#"s! =:!s" !n"o 4?
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Aun8u" ! $#!ns6u##&%o 7o6o $&"m7o %"s%" 8u" s" $u:o 6ons$!n6&! %" "s$! :'! %"
6on$!m&n!6&9n 7o# !6#&l!m&%! "n um!nos@ ! $#!:s %" !l&m"n$os 8u" &n)"#&mos %&!#&os@ "l
!:!n6" 6&"n$'+&6o ! s&%o mu( #>7&%o "n "l %"s!##ollo %" m$o%os +!6$&-l"s ! l! o#! %"
%&sm&nu ( "l&m&n!# 6!n$&%!%"s %" !6#&l!m&%! 7!#! #"%u6 los #&"s)os %" s!lu% 8u"
7u"%"n !+"6$!#nos
F!l$!n %!$os %" :"n$!s !nu!l"s =-us8u"n "n 7!)&n!s %" "m7#"s!s o "n !%u!n!@ (! 8u"
s!-"n 8u" "n 6&l" $- l! us!n?
CAPITULO 2
CONCLUSIONES
S& -&"n l! !s7!#!)&n!s! no 7os"" un! !m7l&! :!#&"%!% %" !7l&6!6&on"s@ "s$o no 8u&"#"
%"6 8u" no s"! &m7o#$!n$" R"s7"6$o !l uso $"#!7u$&6o 6on$#! l! l"u6"m&! $o%!:'! !(
un l!#)o 6!m&no 7o# #"6o##"# S&n "m-!#)o !6$u!lm"n$" "s un! %" l!s !l$"#n!$&:!s m>s
"+"6$&:!s 6on$#! l! l"u6"m&! l&n+o-l>s$&6! !)u%! ( un! "s$#!$")&! !l$!m"n$" #"n$!-l" 6on$#!
l! !6#&l!m&%! "n !l&m"n$os@ l" ! %!%o #"l":!n6&! ! "s$! "n*&m! 8u" 7o%#'! "n6on$#!#
nu":!s !7l&6!6&on"s@ 6omo ! %"mos$#!%o l! 7!$"n$" #"+"#"n$" ! su uso "n l! &n%us$#&!
7"$#ol"#!
S& o$#!s !7l&6!6&on"s@ 6omo l! m"n6&on!%! "n 7o*os 7"$#ol'+"#os@ 6om&"n*!n ! s"#
u$&l&*!%!s s" 7o%#'! :"# un !um"n$o "n l! %"m!n%! %" l! !s7!#!)&n!s! A%"m>s
!7l&6!6&9n %" !s7!#!)&n!s! "n l! &n%us$#&! !l&m"n$!#&! 7!#! l! "l&m&n!6&9n %" l! 6!us!
6>n6"# %" !6#&l!m&%! %" los !l&m"n$os !l o#no ! s&%o uno %" los %"s6u-#&m&"n$os
"m&n"n$"s %" l! 7o6! mo%"#n! Po# lo $!n$o s" n"6"s&$! mu6o m>s $&"m7o 7!#!
"7lo#!# !6"#6! los 7os&-l"s usos %" "s$! &n6#"'-l" "n*&m!
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REFERENCIAS
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. B#oom"@ , D // E:&%"n6" $!$ $" L!s7!#!)&n!s" !6$&:&$( o+ )u&n"! 7&) s"#um
&s #"s7ons&-l" +o# &$s !n$&l(m7om! "++"6$s N!$u#" /////4 ///5 2
2 C!7u!no@ E A@ F"##&)no@ I A6!m7!@ A S"#7"n@ W AX!#@ V GYHm"n@ "$ !l .00
E++"6$ o+ +lou# $(7" on M!&ll!#% #"!6$&on !n% !6#(l!m&%" +o#m!$&on %u#&n) $o!s$&n) o+-#"!% 6#&s7 mo%"l s(s$"ms !n% m&$&)!$&on s$#!$")&"s Foo% R"s"!#6 In$"#n!$&on!l@
4. @ 77 /.5/20.
4 C"%!#@ J@ , S6;!#$* / P#o%u6$&on o+ LAs7!#!)&n!s" II -(
Es6"#&6&! 6ol& , B!6$"#&ol .042.04
5 D" Bo"#@ L CEM J""#"m!ns@ RB M"&m! .0/4 R"%u6$&on o+ !6#(l!m&%"
+o#m!$&on &n -!H"#( 7#o%u6$s -( !77l&6!$&on o+As7"#)&llus N&)"# !s7!#!)&n!s" In
SP C!u:!&n@ LS 1oun) !n% S S!lmon Us&n) C"#"!l S6&"n6" !n% T"6nolo)( +o#
$" B"n"+&$ o+ Consum"#s@ 7740/405 UH oo%"!% Pu-l&s&n)
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D" Mou#!@ M E@ Mo#!&s A@ S&l:! G@ L!#! .004 P#o%u6$&on o+ L!s7!#!)&n!s" -(
F&l!m"n$ous Fun)& M"mo#&!s Do Ins$&$u$o Os;!l%o C#u* =5? 44.
Dunn@ , R"s"!#6 &n -#&"+ ,un" /@ .004 =6&$"% .. A7#&l .0/5? A:!&l!-l" +#om
Z;;;+oo%m!nu+!6$u#"6ouH[n";s[+ulls$o#(77[!&%[/[R"s"!#6 &n -#&"+$ml\
E)u&)u#"n ,M@ Pu& CJ Bon" M!##o; n"6#os&s !n% $#om-o$&6 6om7l&6!$&ons &n
6&l%oo% !6u$" l(m7o-l!s$&6 l"uH"m&! M"% P"%&!$# On6ol /..050
G!ll!)"# M P@ M!#s!ll R D ( &lson R =/? As7!#!)&n!s" !s ! %#u) +o#$#"!$m"n$ o+ !6u$" l(m7o-l!s$&6 l"uH!"m&! Ess!(s &n -&o6"m&s$#( .4 /40
/0 Gu7$!@ D S =.0/.? P!$"n$" n US.0/.0.55 A/ US
// Go;!#% C =/4? Patente n US 52/00 A US
/. Im!%! A@ I)!#!s& S@ N!H!!m! K " Isono M =/2? As7!#!)&n!s" !n% )lu$!m&n!s"
!6$&:&$&"s o+ m&6#oo#)!n&sms , G"n M&6#o-&ol 5
/2 Ku&lm!n@ M L@ &lms .00 S!+"$( o+ $" "n*(m" !s7!#!)&n!s"@ ! m"!ns o+
#"%u6$&on o+ !6#(l!m&%" &n +oo% To&6olo)( L"$$"#s .25 S/S/ Els":&"#
/4 K"!$&n) M,@ Jolm"s R@ L"#n"# S@ Jo DJ /2 L!s7!#!)&n!s" !n% PEG
!s7!#!)&n!s" P!s$@ 7#"s"n$ !n% +u$u#"L"uH L(m7om! /0 /52/5
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/5 Kon%#!$@ E /4 Com7!#!$&:" ":!lu!$&on o+ $" 6l&n&6!l "++&6!6( o+ 2 7#"7!#!$&ons
o+ L!s7!#!)&n!s" +#om Es"#&6&! 6ol& An$&-&o$&H&=Mos6o;?.=? 5.52/
/ M!s-u#n@ L T@ !n% , C #&s$on /4 Tumo# &n&-&$&on "++"6$ o+ L!s7!#!)&n!s"+#om Es6"#&6&! 6ol& A#6 B&o6"m B&o7(s /05450 45.
/ M"##(m!n R@ S$":"nson KE@ Gos$&6 ,@ N"u-"#) D@ O]B#&!n ,@ S!ll!n SE@ "$ !l
As7!#!)&n!s" !sso6&!$"% m("losu77#"ss&on !n% "++"6$ on %os&n) o+ o$"#
6"mo$"#!7"u$&6 !)"n$s &n 6&l%oo% !6u$" l(m7o-l!s$&6 l"uH"m&! P"%&!$#&6
Bloo% C!n6"# .0/.5.5
/ M&$6"ll I@ Joo)"n%oo#n J@ G&l"s AR /4 In6#"!s"% "n%o)"nous $#om-&n
)"n"#!$&on &n 6&l%#"n ;&$ !6u$" l(m7o-l!s$&6 l"uH"m&! #&sH o+ $#om-o$&6
6om7l&6!$&ons &n L !s7!#!)&n!s" &n%u6"% !n$&$#om-&n III %"+&6&"n6(Bloo% 2 2
2/
/ Mo#!l"s@ F C!7u!no@ E !n% Fo)l&!no@ V .00 Ann N1 A6!% S6& //. /00
.0 No:o*(m"s .00 No:o*(m"s !n% DSM l!un6 !6#(l!m&%" +&)$"#s C"m&6!l !n%
En)&n""#&n) N";s 5 .0./
./ O#$un .0/. L!s !s7!#!)&n!s!s %" m&6#oo#)!n&smos ( su uso
6l'n&6o BEB / /5
.. P!#H@ 1 K@ A-u6o;sH&@ A@ D!:&s@ S !n% D!:&s@ F // P!#m!6olo)( o+
E"#&6&! 6ol&L!s7!#!)&n!s" 7ol("$(l"n" )l(6ol !%%u6$ An$&6!n6"# R"s 22
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.2 P!:"ss& A@ E V =.0/4? Es$&m!$" o+ A6#(l!m&%" In$!H" +#om Co++"" !n% J"!l$ R&sH
Ass"ssm"n$ In V P#""%(@ Co++"" &n J"!l$ !n% D&s"!s" P#":"n$&on@ 77 5554
N"; 1o#H A6!%"m&6 P#"ss
.4 P"%#"s6&@ F K@ K!!6H@ K G#!n-( .00 T" "++"6$ o+ !s7!#!)&n!s" on !6#(l!m&%"
+o#m!$&on &n F#"n6 +#&"s Foo% C"m&s$#(@ /0@ 77 22.
.5 P"$"#son@ RE C&")l"#@ A / LAs7!#!)&n!s" P#o%u6$&on -( V!#&ous B!6$"#&!
A77l&"% M&6#o-&olo)( / .20
. R"(nol%s DR@ T!(lo# , /2 T" Fun)!l Jolomo#7 A Cons&%"#!$&on o+ M&$o$&6
M"&o$&6 !n% Pl"omo#7&6 S7"6&!$&on@ CAB In$"#n!$&on!l@ !ll&n)+o#%@ UK
. Ro-"#$s@ ,@ ,M J&ll =/? Patente n!" #$1$%#& A. !".
".S6#"( D@ S7"&$"l K@ L!n:"#sK!m&nsH( C@ G"#ss ,@ Mo#u6H" A@ Boos , F&:" 1"!#C"n$"# S$u%( o+ !s7#!)&n!s" $"#!7( ;&$&n $" ALLBFM .000 $#&!l P"%&!$#&6
Bloo% C!n6"# .0//524
. S&n!@ R JR@ S&n) !n% SK@ ,! .0/2 M&6#o-&!l LAs7!#!)&n!s! P#"s"n$ !n%
+u$u#" P#os7"6$&:" I,IRSET . 02/05/
20 T!#"H" E@ R(%-"#) P@ K!#lsson P@ E#&Hsson S@ TY#n8:&s$ M An!l(s&s o+ !6#(l!m&%"@
! 6!#6&no)"n +o#m"% &n "!$"% +oo%s$u++s , A)#&6 Foo% C"m .00.504500
2/ V!n)@ J K@ Pu%"# A@ Gol%-"l6 .0/4 P!$"n$" n O .0/4//25 A/
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2. V"#m!@ N K@ Kum!# G@ K!u# !n% S An!n% .00 LAs7!#!)&n!s" A P#om&s&n)
C"mo$"#!7"u$&6 A)"n$ C#&$&6!l R":&";s &n B&o$"6nolo)(@ . 45.
22 !n) B@ R"ll&n) MV@ S$o#m MC@ oo MJ@ R&-"o R@ Pu& CJ@ J!H L, E:!lu!$&on o+
&mmunolo)&6 6#oss #"!6$&on o+ !s$&!s7!#!)&n!s" !n$&-o%&"s &n !6u$" l(m7o-l!s$&6
l"uH"m&! =ALL? !n% l(m7om! 7!$&"n$s L"uH"m&! .002//52
BIBLIOGRAFIA
Eu#o7"!n Comm&ss&on COMMISSION RECOMMENDATION o+ /0/.0// on
&n:"s$&)!$&ons &n$o $" l":"ls o+ !6#(l!m&%" &n +oo% A:!&l!-l" on
$$7[["6"u#o7!"u[+oo%[+oo%[6"m&6!ls!+"$([6on$!m&n!n$s[#"6omm"n%!$&on^/00/.0//^!
6#(l!m&%"^+oo%^"n7%+ =on l&n"@ A66"ss"% .0/5 M!# .5?
US Foo% !n% D#u) A%m&n&s$#!$&on A6#(l!m&%"@ Fu#!n@ !n% $" FDA A:!&l!-l"
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/ #":&s$!s %" 25 #"+"#"n6&!s4@5 Z 0
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ANE3OS
ANE3O /
MECANISMO DE ACCION ASPARAGINASA
F&)u#! / Es8u"m! m"6!n&smo %" #"!66&9n !s7!#!)&n!s!
Fu"n$" N""l!m V"#m!@ Kul%""7 Kum!# !n% Gu#noo# K!u#@ Sn" An!n% LAs7!#!)&n!s"
A P#om&s&n) C"mo$"#!7"u$&6 A)"n$
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ANE3O .
DIFERENTES FUENTES BACTERIANAS PARA ASPARAGINASA
-
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1!
T!-l! / D&+"#"n$"s Fu"n$"s -!6$"#&!n!s 7!#! !s7!#!)&n!s!Fu"n$" REP"$"#son !n% AC&")l"# L!s7!#!)&n!s" P#o%u6$&on -( V!#&ous B!6$"#&!
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ANE3O 2
EVOLUCION TEMPORAL DE LA APARACION DE ASPARAGINASA II
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